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ctsk  (Boster Bio)


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    Structured Review

    Boster Bio ctsk
    LFS impedes osteoclast differentiation of BMDM in vitro. (A, B) Representative images of TRAP staining of RANKL‐induced BMDM with different concentration of LFS (0, 1, and 5 μM) and quantitative analysis. Scale bar = 100 μm. (C, D) Representative images of F‐actin ring staining (phalloidin, red) and nuclei (DAPI, blue) of BMDMs, and subsequent quantitative analysis of osteoclast area and the number of nuclei per osteoclast. Scale bar = 50 μm. (E–J) Relative mRNA expressions of osteoclast differentiation related genes of BMDM with LFS (0, 1, and 5 μM) by qPCR. (K–O) Representative western blot images of <t>NFATC1,</t> <t>MMP9,</t> <t>CTSK,</t> ATP6V0D2 and β‐TUBULIN of BMDM with LFS and respective quantitative analysis. Data are presented as means ± SD of 3 independent experiments; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
    Ctsk, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ctsk/product/Boster Bio
    Average 93 stars, based on 15 article reviews
    ctsk - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "Sulforaphene Mitigates Periodontitis by NRF2‐Dependent Regulation of P. gingivalis ‐Induced Inflammatory Response and Bone Homeostasis"

    Article Title: Sulforaphene Mitigates Periodontitis by NRF2‐Dependent Regulation of P. gingivalis ‐Induced Inflammatory Response and Bone Homeostasis

    Journal: Food Science & Nutrition

    doi: 10.1002/fsn3.71319

    LFS impedes osteoclast differentiation of BMDM in vitro. (A, B) Representative images of TRAP staining of RANKL‐induced BMDM with different concentration of LFS (0, 1, and 5 μM) and quantitative analysis. Scale bar = 100 μm. (C, D) Representative images of F‐actin ring staining (phalloidin, red) and nuclei (DAPI, blue) of BMDMs, and subsequent quantitative analysis of osteoclast area and the number of nuclei per osteoclast. Scale bar = 50 μm. (E–J) Relative mRNA expressions of osteoclast differentiation related genes of BMDM with LFS (0, 1, and 5 μM) by qPCR. (K–O) Representative western blot images of NFATC1, MMP9, CTSK, ATP6V0D2 and β‐TUBULIN of BMDM with LFS and respective quantitative analysis. Data are presented as means ± SD of 3 independent experiments; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
    Figure Legend Snippet: LFS impedes osteoclast differentiation of BMDM in vitro. (A, B) Representative images of TRAP staining of RANKL‐induced BMDM with different concentration of LFS (0, 1, and 5 μM) and quantitative analysis. Scale bar = 100 μm. (C, D) Representative images of F‐actin ring staining (phalloidin, red) and nuclei (DAPI, blue) of BMDMs, and subsequent quantitative analysis of osteoclast area and the number of nuclei per osteoclast. Scale bar = 50 μm. (E–J) Relative mRNA expressions of osteoclast differentiation related genes of BMDM with LFS (0, 1, and 5 μM) by qPCR. (K–O) Representative western blot images of NFATC1, MMP9, CTSK, ATP6V0D2 and β‐TUBULIN of BMDM with LFS and respective quantitative analysis. Data are presented as means ± SD of 3 independent experiments; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Techniques Used: In Vitro, Staining, Concentration Assay, Western Blot



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    LFS impedes osteoclast differentiation of BMDM in vitro. (A, B) Representative images of TRAP staining of RANKL‐induced BMDM with different concentration of LFS (0, 1, and 5 μM) and quantitative analysis. Scale bar = 100 μm. (C, D) Representative images of F‐actin ring staining (phalloidin, red) and nuclei (DAPI, blue) of BMDMs, and subsequent quantitative analysis of osteoclast area and the number of nuclei per osteoclast. Scale bar = 50 μm. (E–J) Relative mRNA expressions of osteoclast differentiation related genes of BMDM with LFS (0, 1, and 5 μM) by qPCR. (K–O) Representative western blot images of <t>NFATC1,</t> <t>MMP9,</t> <t>CTSK,</t> ATP6V0D2 and β‐TUBULIN of BMDM with LFS and respective quantitative analysis. Data are presented as means ± SD of 3 independent experiments; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
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    Image Search Results


    Gene expression of osteoclast cells cultured indirectly in conditioned media with i-PRF, materials and materials with i-PRF on day 10. A ACP5 (TRAP); B CTSK (cathepsin K); C TNFRSF11A (RANK); D CA2 (carbonic anhydrase II). Each symbol (Δ; □; O) represents a separate PBMC donor. BT- Biotiss, Collacone, GS – Geistlich, Bio-Oss.® Collagen, TK – Teknimed, CeraForm p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****)

    Journal: BMC Oral Health

    Article Title: Material-dependent effects of injectable platelet rich-fibrin on growth factor release, inflammation, and osteoclast activity: an in vitro study

    doi: 10.1186/s12903-025-07487-w

    Figure Lengend Snippet: Gene expression of osteoclast cells cultured indirectly in conditioned media with i-PRF, materials and materials with i-PRF on day 10. A ACP5 (TRAP); B CTSK (cathepsin K); C TNFRSF11A (RANK); D CA2 (carbonic anhydrase II). Each symbol (Δ; □; O) represents a separate PBMC donor. BT- Biotiss, Collacone, GS – Geistlich, Bio-Oss.® Collagen, TK – Teknimed, CeraForm p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****)

    Article Snippet: CTSK , Hs00166156_m1 , , .

    Techniques: Gene Expression, Cell Culture

    LFS impedes osteoclast differentiation of BMDM in vitro. (A, B) Representative images of TRAP staining of RANKL‐induced BMDM with different concentration of LFS (0, 1, and 5 μM) and quantitative analysis. Scale bar = 100 μm. (C, D) Representative images of F‐actin ring staining (phalloidin, red) and nuclei (DAPI, blue) of BMDMs, and subsequent quantitative analysis of osteoclast area and the number of nuclei per osteoclast. Scale bar = 50 μm. (E–J) Relative mRNA expressions of osteoclast differentiation related genes of BMDM with LFS (0, 1, and 5 μM) by qPCR. (K–O) Representative western blot images of NFATC1, MMP9, CTSK, ATP6V0D2 and β‐TUBULIN of BMDM with LFS and respective quantitative analysis. Data are presented as means ± SD of 3 independent experiments; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Journal: Food Science & Nutrition

    Article Title: Sulforaphene Mitigates Periodontitis by NRF2‐Dependent Regulation of P. gingivalis ‐Induced Inflammatory Response and Bone Homeostasis

    doi: 10.1002/fsn3.71319

    Figure Lengend Snippet: LFS impedes osteoclast differentiation of BMDM in vitro. (A, B) Representative images of TRAP staining of RANKL‐induced BMDM with different concentration of LFS (0, 1, and 5 μM) and quantitative analysis. Scale bar = 100 μm. (C, D) Representative images of F‐actin ring staining (phalloidin, red) and nuclei (DAPI, blue) of BMDMs, and subsequent quantitative analysis of osteoclast area and the number of nuclei per osteoclast. Scale bar = 50 μm. (E–J) Relative mRNA expressions of osteoclast differentiation related genes of BMDM with LFS (0, 1, and 5 μM) by qPCR. (K–O) Representative western blot images of NFATC1, MMP9, CTSK, ATP6V0D2 and β‐TUBULIN of BMDM with LFS and respective quantitative analysis. Data are presented as means ± SD of 3 independent experiments; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

    Article Snippet: Following blocked with blocking buffer (Beyotime, China), the membranes were incubated overnight at 4°C with primary antibodies specific to SP7 (Catalogue number: ab209484, Abcam, USA), OCN (Catalogue number: b0822, Santa Cruz Biotechnology, USA), NFATC1 (Catalogue number: d0522, Santa Cruz Biotechnology, USA), IL‐1β (Catalogue number: A19635, ABclonal Technology, China), IL‐6 (Catalogue number: A0286, ABclonal Technology, China), CTSK (Catalogue number: PB9856, Bosterbio, China), MMP9 (Catalogue number: ab76003, Abcam, USA), ATP6V0D2 (Catalogue number: 333641‐ap, Proteintech, China), TRAP (Catalogue number: ab133238, Abcam, USA), and β‐TUBULIN (Catalogue number: M20045F, Abmart, China) at 4°C overnight.

    Techniques: In Vitro, Staining, Concentration Assay, Western Blot